Cadmium-responsive thiols in the ectomycorrhizal fungus Paxillus involutus.
Identifieur interne : 002625 ( Main/Exploration ); précédent : 002624; suivant : 002626Cadmium-responsive thiols in the ectomycorrhizal fungus Paxillus involutus.
Auteurs : Mikael Courbot [France] ; Laurent Diez ; Roberta Ruotolo ; Michel Chalot ; Pierre LeroySource :
- Applied and environmental microbiology [ 0099-2240 ] ; 2004.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN (MeSH), Basidiomycota (effets des médicaments et des substances chimiques), Basidiomycota (génétique), Basidiomycota (métabolisme), Cadmium (métabolisme), Cadmium (pharmacologie), Chromatographie en phase liquide à haute performance (MeSH), Données de séquences moléculaires (MeSH), Glutathion (métabolisme), Milieux de culture (MeSH), Mycorhizes (MeSH), Métalloprotéines (métabolisme), Métallothionéine (métabolisme), Phytochélatines (MeSH), Thiols (métabolisme).
- MESH :
- effets des médicaments et des substances chimiques : Basidiomycota.
- génétique : Basidiomycota.
- métabolisme : Basidiomycota, Cadmium, Glutathion, Métalloprotéines, Métallothionéine, Thiols.
- pharmacologie : Cadmium.
- Analyse de séquence d'ADN, Chromatographie en phase liquide à haute performance, Données de séquences moléculaires, Milieux de culture, Mycorhizes, Phytochélatines.
English descriptors
- KwdEn :
- Basidiomycota (drug effects), Basidiomycota (genetics), Basidiomycota (metabolism), Cadmium (metabolism), Cadmium (pharmacology), Chromatography, High Pressure Liquid (MeSH), Culture Media (MeSH), Glutathione (metabolism), Metalloproteins (metabolism), Metallothionein (metabolism), Molecular Sequence Data (MeSH), Mycorrhizae (MeSH), Phytochelatins (MeSH), Sequence Analysis, DNA (MeSH), Sulfhydryl Compounds (metabolism).
- MESH :
- chemical , metabolism : Cadmium, Glutathione, Metalloproteins, Metallothionein, Sulfhydryl Compounds.
- drug effects : Basidiomycota.
- genetics : Basidiomycota.
- metabolism : Basidiomycota.
- chemical , pharmacology : Cadmium.
- Chromatography, High Pressure Liquid, Culture Media, Molecular Sequence Data, Mycorrhizae, Phytochelatins, Sequence Analysis, DNA.
Abstract
Molecular and cellular mechanisms underlying the sustained metal tolerance of ectomycorrhizal fungi are largely unknown. Some of the main mechanisms involved in metal detoxification appear to involve the chelation of metal ions in the cytosol with thiol-containing compounds, such as glutathione, phytochelatins, or metallothioneins. We used an improved high-performance liquid chromatography method for the simultaneous measurement of thiol-containing compounds from cysteine and its derivatives (gamma-glutamylcysteine, glutathione) to higher-molecular-mass compounds (phytochelatins). We found that glutathione and gamma-glutamylcysteine contents increased when the ectomycorrhizal fungus Paxillus involutus was exposed to cadmium. An additional compound with a 3-kDa molecular mass, most probably related to a metallothionein, increased drastically in mycelia exposed to cadmium. The relative lack of phytochelatins and the presence of a putative metallothionein suggest that ectomycorrhizal fungi may use a different means to tolerate heavy metals, such as Cd, than do their plant hosts.
DOI: 10.1128/AEM.70.12.7413-7417.2004
PubMed: 15574943
PubMed Central: PMC535167
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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sortKey="Ruotolo, Roberta" sort="Ruotolo, Roberta" uniqKey="Ruotolo R" first="Roberta" last="Ruotolo">Roberta Ruotolo</name></author><author><name sortKey="Chalot, Michel" sort="Chalot, Michel" uniqKey="Chalot M" first="Michel" last="Chalot">Michel Chalot</name></author><author><name sortKey="Leroy, Pierre" sort="Leroy, Pierre" uniqKey="Leroy P" first="Pierre" last="Leroy">Pierre Leroy</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15574943</idno><idno type="pmid">15574943</idno><idno type="doi">10.1128/AEM.70.12.7413-7417.2004</idno><idno type="pmc">PMC535167</idno><idno type="wicri:Area/Main/Corpus">002559</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002559</idno><idno type="wicri:Area/Main/Curation">002559</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002559</idno><idno 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last="Diez">Laurent Diez</name></author><author><name sortKey="Ruotolo, Roberta" sort="Ruotolo, Roberta" uniqKey="Ruotolo R" first="Roberta" last="Ruotolo">Roberta Ruotolo</name></author><author><name sortKey="Chalot, Michel" sort="Chalot, Michel" uniqKey="Chalot M" first="Michel" last="Chalot">Michel Chalot</name></author><author><name sortKey="Leroy, Pierre" sort="Leroy, Pierre" uniqKey="Leroy P" first="Pierre" last="Leroy">Pierre Leroy</name></author></analytic><series><title level="j">Applied and environmental microbiology</title><idno type="ISSN">0099-2240</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (drug effects)</term><term>Basidiomycota (genetics)</term><term>Basidiomycota (metabolism)</term><term>Cadmium (metabolism)</term><term>Cadmium (pharmacology)</term><term>Chromatography, High Pressure Liquid (MeSH)</term><term>Culture Media (MeSH)</term><term>Glutathione (metabolism)</term><term>Metalloproteins (metabolism)</term><term>Metallothionein (metabolism)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycorrhizae (MeSH)</term><term>Phytochelatins (MeSH)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Sulfhydryl Compounds (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN (MeSH)</term><term>Basidiomycota (effets des médicaments et des substances chimiques)</term><term>Basidiomycota (génétique)</term><term>Basidiomycota (métabolisme)</term><term>Cadmium (métabolisme)</term><term>Cadmium (pharmacologie)</term><term>Chromatographie en phase liquide à haute performance (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Glutathion (métabolisme)</term><term>Milieux de culture (MeSH)</term><term>Mycorhizes (MeSH)</term><term>Métalloprotéines (métabolisme)</term><term>Métallothionéine (métabolisme)</term><term>Phytochélatines (MeSH)</term><term>Thiols (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cadmium</term><term>Glutathione</term><term>Metalloproteins</term><term>Metallothionein</term><term>Sulfhydryl Compounds</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="métabolisme" 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cellular mechanisms underlying the sustained metal tolerance of ectomycorrhizal fungi are largely unknown. Some of the main mechanisms involved in metal detoxification appear to involve the chelation of metal ions in the cytosol with thiol-containing compounds, such as glutathione, phytochelatins, or metallothioneins. We used an improved high-performance liquid chromatography method for the simultaneous measurement of thiol-containing compounds from cysteine and its derivatives (gamma-glutamylcysteine, glutathione) to higher-molecular-mass compounds (phytochelatins). We found that glutathione and gamma-glutamylcysteine contents increased when the ectomycorrhizal fungus Paxillus involutus was exposed to cadmium. An additional compound with a 3-kDa molecular mass, most probably related to a metallothionein, increased drastically in mycelia exposed to cadmium. The relative lack of phytochelatins and the presence of a putative metallothionein suggest that ectomycorrhizal fungi may use a different means to tolerate heavy metals, such as Cd, than do their plant hosts.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15574943</PMID><DateCompleted><Year>2005</Year><Month>02</Month><Day>15</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0099-2240</ISSN><JournalIssue CitedMedium="Print"><Volume>70</Volume><Issue>12</Issue><PubDate><Year>2004</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Applied and environmental microbiology</Title><ISOAbbreviation>Appl Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>Cadmium-responsive thiols in the ectomycorrhizal fungus Paxillus involutus.</ArticleTitle><Pagination><MedlinePgn>7413-7</MedlinePgn></Pagination><Abstract><AbstractText>Molecular and cellular mechanisms underlying the sustained metal tolerance of ectomycorrhizal fungi are largely unknown. Some of the main mechanisms involved in metal detoxification appear to involve the chelation of metal ions in the cytosol with thiol-containing compounds, such as glutathione, phytochelatins, or metallothioneins. We used an improved high-performance liquid chromatography method for the simultaneous measurement of thiol-containing compounds from cysteine and its derivatives (gamma-glutamylcysteine, glutathione) to higher-molecular-mass compounds (phytochelatins). We found that glutathione and gamma-glutamylcysteine contents increased when the ectomycorrhizal fungus Paxillus involutus was exposed to cadmium. An additional compound with a 3-kDa molecular mass, most probably related to a metallothionein, increased drastically in mycelia exposed to cadmium. The relative lack of phytochelatins and the presence of a putative metallothionein suggest that ectomycorrhizal fungi may use a different means to tolerate heavy metals, such as Cd, than do their plant hosts.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Courbot</LastName><ForeName>Mikael</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Interactions Arbres/Micro-organismes, Faculté des Sciences et Techniques, Université Henri Poincaré-Nancy I, Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Diez</LastName><ForeName>Laurent</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Ruotolo</LastName><ForeName>Roberta</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Chalot</LastName><ForeName>Michel</ForeName><Initials>M</Initials></Author><Author 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Diez</name><name sortKey="Leroy, Pierre" sort="Leroy, Pierre" uniqKey="Leroy P" first="Pierre" last="Leroy">Pierre Leroy</name><name sortKey="Ruotolo, Roberta" sort="Ruotolo, Roberta" uniqKey="Ruotolo R" first="Roberta" last="Ruotolo">Roberta Ruotolo</name></noCountry><country name="France"><region name="Grand Est"><name sortKey="Courbot, Mikael" sort="Courbot, Mikael" uniqKey="Courbot M" first="Mikael" last="Courbot">Mikael Courbot</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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